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| Registros recuperados: 14 | |
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| Corporeau, Charlotte; Groisillier, Agnes; Jeudy, Alexandra; Barbeyron, Tristan; Fleury, Elodie; Fabioux, Caroline; Czjzek, Mirjam; Huvet, Arnaud. |
| The transforming growth factor (TGF)-beta superfamily is a group of important growth factors involved in multiple processes such as differentiation, cell proliferation, apoptosis and cellular growth. In the Pacific oyster Crassostrea gigas, the oyster gonadal (og) TGF-beta gene was recently characterized through genome-wide expression profiling of oyster lines selected to be resistant or susceptible to summer mortality. Og TGF-beta appeared specifically expressed in the gonad to reach a maximum when gonads are fully mature, which singularly contrasts with the pleiotropic roles commonly ascribed to most TGF-beta family members. The function of og TGF-beta protein in oysters is unknown, and defining its role remains challenging. In this study, we develop a... |
| Tipo: Text |
Palavras-chave: Transforming growth factor-beta; Bacterial expression; In vivo antibody inhibition; Reproduction; Crassostrea gigas; Marine bivalve. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00042/15371/13438.pdf |
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| Harrang, Estelle; Lapegue, Sylvie; Morga, Benjamin; Bierne, Nicolas. |
| Marine bivalves show among the greatest allozyme diversity ever reported in Eukaryotes, putting them historically at the heart of the neutralist-selectionist controversy on the maintenance of genetic variation. Although it is now acknowledged that this high diversity is most probably a simple consequence of a large population size, convincing support for this explanation would require a rigorous assessment of the silent nucleotide diversity in natural populations of marine bivalves, which has not yet been done. This study investigated DNA sequence polymorphism in a set of 37 nuclear loci in wild samples of the flat oyster Ostrea edulis. Silent diversity was found to be only moderate (0.7%), and there was no departure from demographic equilibrium under the... |
| Tipo: Text |
Palavras-chave: Nucleotide; Polymorphism; Marine bivalve; Deleterious; Mutations; Genetic load; Ostrea edulis. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00125/23610/21444.pdf |
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| De Sousa, Joana Teixeira; Milan, Massimo; Pauletto, Marianna; Bargelloni, Luca; Joaquim, Sandra; Matias, Domitilia; Matias, Ana Margarete; Quillien, Virgile; Leitao, Alexandra; Huvet, Arnaud. |
| A microarray-based analysis was performed with the objective of describing genomic features of oocytes and looking for potential markers of oocyte quality in the economically important European clam, Ruditapes decussatus. Oocytes of 25 females from Ria de Aveiro, Western coast of Portugal (40°42′N; 08°40′W) were selected for this study and oocyte quality was estimated by success of D-larval rate under controlled conditions, which appeared to vary from 0 to 95 %. By genome-wide expression profiling with a DNA microarray, 526 probes appeared differentially expressed between two groups representing the largest and smallest value of D larval rates, named good (represented by a mean D-larval yield of 57 ± 22%) and poor (9 ± 5%) quality oocytes. Enrichment... |
| Tipo: Text |
Palavras-chave: Ruditapes decussatus; Oocyte; Developmental success; Gene expression; Marine bivalve; Transcriptomics. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00260/37147/35676.pdf |
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| Huvet, Arnaud; Beguel, Jean-philippe; Pereira Cavaleiro, Nathalia; Thomas, Yoann; Quillien, Virgile; Boudry, Pierre; Alunno-bruscia, Marianne; Fabioux, Caroline. |
| Feeding strategies and digestive capacities can have important implications for variation in energetic pathways associated with ecological and economically important traits, such as growth or reproduction in bivalve species. Here, we investigated the role of amylase in the digestive processes of Crassostrea gigas, using in vivo RNA interference. This approach also allowed us to investigate the relationship between energy intake by feeding and gametogenesis in oysters. Double-stranded (ds)RNA designed to target the two α-amylase genes A and B was injected in vivo into the visceral mass of oysters at two doses. These treatments caused significant reductions in mean mRNA levels of the amylase genes: −50.7% and −59% mRNA A, and −71.9% and −70.6% mRNA B in 15... |
| Tipo: Text |
Palavras-chave: Alpha-Amylase; DsRNA; Gametogenesis; Marine bivalve; Dynamic energy budget. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00271/38239/36422.pdf |
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| Menoud, Mathilde; Van Wynsberge, Simon; Le Moullac, Gilles; Levy, Peva; Andrefouet, Serge; Remoissenet, Georges; Gaertner-mazouni, Nabila. |
| Monitoring gonadmaturation for protandrous and functional hermaphrodite species such as the giant clamTridacna maxima is difficult due to the juxtaposition and relative proportion ofmale and female tissues in the gonad [gonadal sex ratio (GSR)]. Here, the relevance of the widely used gonadosomatic index (GSI) as proxy of giant clam gonad maturation is tested with a large dataset (n = 265). Gonadosomatic index is compared with other indices, namely the proportion of the male part harboring spermatozoids, the proportion of empty oocyte follicles, the mean oocyte diameter, and the oocyte elongation. At gonad scale, high index variability highlighted partial spawning. At individual scale, male and female maturation proxies were contrasted, showing either... |
| Tipo: Text |
Palavras-chave: Giant clam; Marine bivalve; Reproduction; Spawn; Tatakoto atoll; Histology; Tridacna maxima. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00333/44441/44111.pdf |
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| Fabioux, Caroline; Corporeau, Charlotte; Quillien, Virgile; Favrel, Pascal; Huvet, Arnaud. |
| This study investigated the potential of RNA interference, which is technically challenging in bivalve mollusc species, to assess gene function in the oyster Crassostrea gigas. We designed dsRNA targeting the oyster vasa-like gene (Oyvlg), specifically expressed in oyster germ cells. In vivo injection of oyvl-dsRNA into the gonad provokes a knockdown phenotype corresponding to germ cell underproliferation and prematurely arrested meiosis througout the organ. The most severe phenotype observed is sterile. This knockdown phenotype is associated with a decrease in Oyvlg mRNA level of between 39% and 87%, and a strong reduction in OYVLG protein, to an undetectable level. Therefore, Oyvlg appears to be essential for germ cell development in Crassostrea gigas,... |
| Tipo: Text |
Palavras-chave: Vasa; RNAi; Marine bivalve; Germline; Crassostrea gigas. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6641.pdf |
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| Huvet, Arnaud; Fleury, Elodie; Corporeau, Charlotte; Quillien, Virgile; Daniel, Jean-yves; Riviere, Guillaume; Boudry, Pierre; Fabioux, Caroline. |
| We investigated the role of oyster gonadal TGF beta (og-TGF beta) in the reproduction of Crassostrea gigas, using an in vivo RNA interference approach. We designed double-stranded RNA targeting og-TGF beta, which is specifically expressed in the somatic cells surrounding germ cells in the gonad of both male and female oysters. In vivo injection of this og-TGF beta dsRNA into the gonad led to knock-down phenotypes for both sexes, with significant reduction (77.52% relative to controls) of the gonad area, lowered reproductive effort and germ cell under-proliferation. Interestingly, half of the injected females halted their vitellogenesis, since we were only able to observe pre-vitellogenic oocytes. In addition, apoptotic germ cells and haemocytes infiltrated... |
| Tipo: Text |
Palavras-chave: Marine bivalve; Crassostrea gigas; Reproduction; RNA interference; Transforming growth factor-beta. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00087/19794/17486.pdf |
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| Pauletto, Marianna; Segarra, Amelie; Montagnani, Caroline; Quillien, Virgile; Faury, Nicole; Le Grand, Jacqueline; Miner, Philippe; Petton, Bruno; Labreuche, Yannick; Fleury, Elodie; Fabioux, Caroline; Bargelloni, Luca; Renault, Tristan; Huvet, Arnaud. |
| Double-stranded RNA (dsRNA)-mediated genetic interference (RNAi) is a widely used reverse genetic tool for determining the loss-of-function phenotype of a gene. Here, the possible induction of an immune response by long dsRNA was tested in a marine bivalve (Crassostrea gigas), as well as the specific role of the subunit 2 of the nuclear factor kappa B inhibitor (I kappa B2). This gene is a candidate of particular interest for functional investigations in the context of oyster mass mortality events, as Cg-I kappa B2 mRNA levels exhibited significant variation depending on the amount of ostreid herpesvirus 1 (OsHV-1) DNA detected. In the present study, dsRNAs targeting Cg-I kappa B2 and green fluorescent protein genes were injected in vivo into oysters... |
| Tipo: Text |
Palavras-chave: Anti-viral response; RNA interference; Inhibitor of NF-kappa B; Marine bivalve; Ostreid herpesvirus 1. |
| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00409/52011/52698.pdf |
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| Gardon, Tony; Morvan, Lucie; Huvet, Arnaud; Quillien, Virgile; Soyez, Claude; Le Moullac, Gilles; Le Luyer, Jeremy. |
| A combined approach integrating bioenergetics and major biological activities is essential to properly understand the impact of microplastics (MP) on marine organisms. Following experimental exposure of polystyrene microbeads (micro-PS of 6 and 10 μm) at 0.25, 2.5, and 25 μg L−1, which demonstrated a dose-dependent decrease of energy balance in the pearl oyster Pinctada margaritifera, a transcriptomic study was conducted on mantle tissue. Transcriptomic data helped us to decipher the molecular mechanisms involved in P. margaritifera responses to micro-PS and search more broadly for effects on energetically expensive maintenance functions. Genes related to the detoxification process were impacted by long-term micro-PS exposure through a decrease in... |
| Tipo: Text |
Palavras-chave: Microbeads; Marine bivalve; Differentially expressed genes; Stress response; Energy-limited tolerance. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00640/75213/75351.pdf |
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| Guevelou, Eric; Huvet, Arnaud; Sussarellu, Rossana; Milan, Massimo; Guo, Xinmei; Li, Li; Zhang, Guofan; Quillien, Virgile; Daniel, Jean-yves; Quere, Claudie; Boudry, Pierre; Corporeau, Charlotte. |
| AMP-activated protein kinase α (AMPKα) is a key regulator of energy balance in many model species during hypoxia. In a marine bivalve, the Pacific oyster Crassostrea gigas, we analyzed the protein content of adductor muscle in response to hypoxia during 6 h. In both smooth and striated muscles, the amount of full-length AMP-activated protein kinase α (AMPKα) remained unchanged during hypoxia. However, hypoxia induced a rapid and muscle-specific response concerning truncated isoforms of AMPKα. In the smooth muscle, a truncated isoform of AMPKα was increased from 1 to 6 h of hypoxia, and was linked with accumulation of AKT kinase, a key enzyme of the insulin signaling pathway which controls intracellular glucose metabolism. In this muscle, aerobic metabolism... |
| Tipo: Text |
Palavras-chave: Marine bivalve; Crassostrea gigas; Hypoxia; AMP-activated protein kinase; Alternative splicing; Adductor muscle. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00119/22998/21397.pdf |
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| Guevelou, Eric; Huvet, Arnaud; Galindo-sanchez, Clara E.; Milan, Massimo; Quillien, Virgile; Daniel, Jean-yves; Quere, Claudie; Boudry, Pierre; Corporeau, Charlotte. |
| The hermaphrodite Pacific oyster Crassostrea gigas displays a high energy allocation to reproduction. We studied the expression of AMP-activated protein kinase (AMPK) during gametogenesis in the gonad and characterized the mRNA sequences of the AMPK subunits: the AMPK alpha mRNA sequence was previously characterized; we identified AMPK beta, AMPK gamma, and mRNAs of putative AMPK-related targets following bioinformatics mining on existing genomic resources. We analyzed the mRNA expression of the AMPK alpha, beta, and gamma subunits in the gonads of male and female oysters through a reproductive cycle, and we quantified the mRNA expression of genes belonging to fatty acid and glucose metabolism. AMPK alpha mRNA levels were more abundant in males at the... |
| Tipo: Text |
Palavras-chave: AMP-activated protein kinase; Crassostrea gigas; Gametogenesis; Marine bivalve; Reproduction. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00168/27911/26470.pdf |
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| Gardon, Tony; Huvet, Arnaud; Paul-pont, Ika; Cassone, Anne-laure; Koua, Manaarii Sham; Soyez, Claude; Jezequel, Ronan; Receveur, Justine; Le Moullac, Gilles. |
| Pearl-farming leads to significant plastic pollution in French Polynesia (FP) as the end of life of most farming gear is currently poorly managed. Plastic debris released into the aquatic environment accumulates, with potentially detrimental effects on the lagoon ecosystem and pearl oyster Pinctada margaritifera, a species of ecological, commercial and social value. Here, we tested the effects of leachates from new (N) and aged (A) plastic pearl-farming gear (spat collector and synthetic rope) obtained after 24 h and 120 h incubation, on the embryo-larval development of the pearl oyster using an in-vitro assay. Embryos were exposed for 24 h and 48 h to a negative control (0) and the leachate from 0.1, 1, 10 and 100 g of plastic. L−1. After 24 h exposure to... |
| Tipo: Text |
Palavras-chave: Aquaculture; Synthetic materials; Hazardous chemicals; Toxicity; Marine bivalve; Early development. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00627/73917/73257.pdf |
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| Di Poi, Carole; Costil, Katherine; Bouchart, Valerie; Halm-lemeille, Marie-pierre. |
| Despite a growing scientific attention on ecological impact of emerging pollutants (EPs) such as pharmaceuticals, personal care products, and pesticides, knowledge gaps remain regarding mixture toxicity and effects on aquatic organisms. Several EPs were screened in seawater (Normandy, France), and the ecotoxicity of five compounds, chosen on their occurrence in ecosystems and use worldwide, was assessed and were the biocides methylparaben (MP) and triclosan (TCS), a pesticide degradation product (AMPA), and the pharmaceuticals venlafaxine (VEN) and carbamazepine (CBZ). The acute or sub-chronic toxicity, alone or in binary/ternary mixtures of three of them (CBZ, AMPA, and MP), was assessed on one marine and two freshwater organisms: Crassostrea gigas,... |
| Tipo: Text |
Palavras-chave: Pharmaceuticals; Personal care products; Pesticides; Mixture and single-compound toxicity; Freshwater species; Marine bivalve. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00389/50004/52260.pdf |
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| Registros recuperados: 14 | |
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